FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. BUV496 has been exclusively developed by BD Life Sciences. BD Horizon™ BUV563 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 560 nm. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. Reagents conjugated to PE, PerCP, PerCP-Cy5.5, APC, and APC-Cy7 tandem fluorochrome can be used on leukocytes from these mouse strains. APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray … It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm. Alexa Fluor® and Texas Red® are registered trademarks, and Pacific Blue™ and Pacific Orange™ are trademarks of Life Technologies Corporation. “CE-IVD”: products intended for in vitro diagnostic use and conforming to European Directive (98/79/EC). It has a 650 nm wavelength absorption maximum and a 660 nm fluorescence emission maximum. After excitation at 337 nm (from a nitrogen laser) the Eu 3+ –TBP complex emits at 580, 620, 650, and 700 nm, the highest peak being at 580 nm. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy. PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. The table below (Table 2) contains data on the peak excitation and emission wavelengths of over 75 selected fluorochromes along with suggested applications and excitation filter cube type. BB515 is a dye that was exclusively developed by BD Biosciences as an additional bright dye for the blue laser. APC-H7 conjugates provide greater stability in light and paraformaldehyde fixatives and have less spillover into the APC channel than APC-Cy7 conjugates. Allow. Excitation Laser Line (nm) Fluorescence Channel Fluorochromes provided by BD Biosciences BD FACSArray™ bioanalyzer Green Diode 532 Yellow PE Far Red PerCP-Cy5.5 PE-Cy7 Red Diode 635 Red APC Alexa Fluor® 647 Infrared BD APC-H7 APC-Cy7 *BD FACSCalibur™ flow cytometry system Argon 488 FL1 Green FITC Alexa Fluor® 488 FL2 Yellow PE To compare APC/Fire™ 750 with other fluorophores on a spectral cytometer, use our Aurora Spectral Analyzer tool. Some dyes like DAPI, PI, etc.. differ in the spectra after binding to the target molecules. It has a broad spectrum of excitation with the main peak at 482 nm, and a long Stokes shift to an emission … APC tetramers are labeled with Streptavidin-Allophycocyanin (SA-APC), excitation 633–635 nm/emission 660–680 nm. Due to similar excitation and emission properties, APC-R700 and Alexa Fluor® 700 cannot be used simultaneously. BV750 is designed for instruments equipped with a 405nm Violet laser and a 750/30 filter. PE or APC) with an acceptor BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. l- 9D* H w R v H R 6 6 D DE ؼ 9 l `[ *_ uI H #0 Dr H R ( b'ʂH p Q .Fq TfˁI[0 H2 ]d 7 R 0 f U C h+@ V$ endstream endobj startxref 0 %%EOF 134 0 obj This dye is significantly brighter than FITC and has less spillover into the PE channel. BD Horizon™ BUV496 is a tandem fluorochrome that combines BD Horizon™ BUV395 and an acceptor dye with an emission max at 491 nm. The tandem dyes APC-Vio 770, APC-Cy7, and APC-H7 all use APC as the donor fluorochrome, showing maximum absorption around 652 nm. It has less Fc receptor–mediated nonspecific staining than PE-Cy5. APC emits fluorescence at 660 nm. Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability. In order to determine the emission spectrum of a particular fluorochrome, the wavelength of maximum absorption (usually the same as the excitation maximum) is determined and the fluorochrome is excited at that wavelength. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, APC-Cy7 and APC-H7 cannot be used simultaneously. APC-H7 conjugates are typically 75% as bright as equivalent APC-Cy7 conjugates. R718 enables the resolution of low expression markers without having to switch to a tandem dye (APC-R700), which can introduce residual emission and resulting SOV/spread into the APC channel. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Alexa Fluor® 700 is a far-red dye that can be excited with a 633–640-nm laser. In the US, call 1-800-4BIORAD (1-800-424-6723). APC-Cy™7 is a tandem fluorochrome that combines APC and a cyanine dye (Cy7). Simply click on the representative spectrum colors to view histograms that represent the absorption and emission spectra for each BD™ fluorochrome. Dye APC (allophycocyanin) Excitation. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage. Please review our cookies policy and configure your cookies for your experience. APC-Cy7 Infrared 650 633, 635, 640 785 †††††† BD™ APC-H7 Infrared 650 633, 635, 640 785 †††††† BD Horizon™ BV786 Infrared 407 405 786 ††† Analyzers Sorters * The excitation laser line represents commonly used lasers that excite the fl uorochrome. BUV732 is designed for instruments equipped with a 355nm UV laser and a 740/35 filter. Efficient excitation of FITC is between 480-500 nm, with lambda max* of 490 nm, The lambda max of FITC emission is at 520 nm, *Lambda max is a wavelength of a spectrum where there is the strongest photon absorption (i.e. For cell types that exhibit high levels of autofluorescence, using a dye with a longer emission wavelength (e.g., APC, APC-Cy7) often provides an excellent signal-to-noise ratio. APC channels.” Ryan Duggan, UC Flow Core Lab Director FITC PE PC7 APC PB CytoFLEX 2 | EVERY vent Matters The CytoFLEX Platform is a revolutionary system presenting optimal excitation and emission, minimizing light loss and maximizing sensitivity. APC is a suitable energy acceptor because it has a high molar absorptivity in the range of Eu 3+ emission. This enables multicolor analysis in conjunction with APC or Alexa Fluor® 647, and APC-H7 or APC-Cy7 reagents. BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. Figure 1: Tandem dyes A. Diagrammatic representation of transfer of energy between donor and acceptor molecules in a tandem dye PE-Cy7. Be sure to also look up the excitation and emission spectra for your dye of choice. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. BUV615 is designed for instruments equipped with a 355nm UV laser and a 610/20 filter. The excitation and emission maxima are nearly identical to those of FITC. However, Alexa Fluor 647 is considered more optimal for intracellular applications. A tandem dye consists of a Emission Excitation: 631 - 671 nm Emission: 640 - 680 nm. Because of its broad absorption range and the fact that its emission spectra are equivalent to APC, PE-Cy5 is not recommended for simultaneous use with APC. Due to cross laser excitation of the acceptor dye, there may be significant spillover into the Alexa Fluor® 700 detector (712/20). Emission ; BP 385/30 BP 469/38 BP 555/30 BP 631/33 … Due to the excitation of the BV421 donor by the 355 nm (UV) laser line, there may be spillover into the BUV737 detector off of the UV laser. For cell types that are not very autofluorescent, such as fresh cells, the improved separation seen with long-wavelength excitation … This dye has been developed exclusively by BD Biosciences as a brighter alternative to Alexa Fluor® 700. In addition alternative laser lines are becoming more affordable so dyes excited by 355 nm and 405 nm lasers are increasing the options for multiplexing. Pacific Blue™ is based on the 6,8-difluoro-7-hydroxycoumarin fluorophore, and is strongly fluorescent, even at neutral pH. FITC is sensitive to pH changes and photobleaching. BD Horizon™ BV750 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with an emission max at 745 nm. → Energy transfer dye excited at ~488 nm with emission maximum at ~670 nm → Also known as PE-Cy®5 → Bright fluorescence → Low compensation against RPE → Significant emission in APC channel with 633 nm excitation → Measured in FL3 channel on … with FITC/PE channels, the same excitation laser will cause emission that will be detected by both the FITC and PE detector, but different excitation lasers for APC… Additionally, the PerCP-Cy5.5 tandem conjugate is not as susceptible to fixative or light instability compared to APC-Cy7 and PE-Cy7. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm. Due to nearly identical excitation and emission properties, APC-Cy7 and APC-H7 cannot be used simultaneously. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. Emission spectra for Vio 770, Cy7, H7, and Alexa Fluor 750 are similar. BD Horizon™ BV480 is part of the BD Horizon Brilliant™ Violet family of dyes. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. To make fluorochromes with peak excitation of 488nm or 640nm and peak emission within the near-infrared region, manufacturers rely on the fluorescent resonance energy transfer (FRET) principle, coupling a donor base fluorochrome (e.g. BB700 is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5. Excitation and emission spectra of Alexa Fluor ® 488, PE, PerCP-Cy5.5, and PE-Cy7 following excitation with the blue laser (488 nm). In addition, tandem conjugates of phycobiliproteins are available to generate a range of emission signals from a single excitation wavelength. This site uses cookies. This site uses cookies to better deliver content. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Allophycocyanin, also known as APC, is typically used for flow cytometry, microarrays, ELISA, and other applications that require high sensitivity but not photostability. BUV805 has been exclusively developed by BD Life Sciences. APC/Fire™ 750 is a novel, more stable, high performance alternative to APC/Cy7. Emission ; BP 385/30 BP 469/38 BP 555/30 BP 631/33 … Due to the close proximity of their emission spectra, there will be significant spillover from BV750 into BV786. Excitation max (nm) 496: Emission max (nm) 785: Recommended for: Low density markers: Incompatible dyes: APC-Cy™7 (on instruments with collinear lasers) Comments: Compatible with green (532 nm) and yellow-green (561 nm) lasers; Tandem dye is sensitive to degradation. R718 has been exclusively developed for BD Life Sciences. Most strikingly, it has two excitation maxima: one at around 488 nm and one at around 561 nm. BUV615 has been exclusively developed by BD Life Sciences. Alexa Fluor® 488 exhibits extraordinary photostability, which makes it highly suitable for fluorescence microscopy. The excitation and emission maxima are nearly identical to those of APC. → Energy transfer dye excited at ~488 nm with emission maximum at ~670 nm → Also known as PE-Cy®5 → Bright fluorescence → Low compensation against RPE → Significant emission in APC channel with 633 nm excitation → Measured in FL3 channel on … PE-Cy™5 is a tandem conjugate that combines phycoerythrin and a cyanine dye. Interestingly, under conditions of 561 nm excitation, there is an appreciable probability that emitted photons will be higher in energy than absorbed photons. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Dye APC (allophycocyanin) Excitation. After excitation at 337 nm (from a nitrogen laser) the Eu 3+ –TBP complex emits at 580, 620, 650, and 700 nm, the highest peak being at 580 nm. BUV563 has been exclusively developed by BD Life Sciences. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage. R-HeNe APC-Cy5.5 633/695 LSRII/Aria/CantoII R-HeNe Alexa700 633/720 Aria R-HeNe APC-Cy7 (H7) 633/785 LSRII/Aria/CantoII Laser Fluorescent Protein Excitation/Emission nm Instrument UV BFP 360440 LSRII UV Sapphire 399/511 LSRII UV T-Sapphire 399/511 LSRII Violet eCFP 436/475 LSRII/Aria/CantoII Violet mCFP 433/476 LSRII/Aria/CantoII BUV805 is designed for instruments equipped with a 355nm UV laser and a 820/60 filter. The emission of light from fluorescein, ranges from 490 nm to 630 nm, and the emission peak is approximately 520 nm. Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. Here are some hints and tips on how to handle tandem dyes: Always protect tandem dyes from light as they are highly susceptible to photobleaching. The carbocyanine-based dye exhibits far-red fluorescence with excitation at 642 nm and emission at 661 nm. The absorption spectrum of a typical fluorochrome is illustrated in Figure 1(a) where the relative intensity of absorption is plotted against the measured wavelength. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength. APC-R700 is a tandem fluorochrome that combines APC with R700, a proprietary organic dye. PE-Cy7 conjugated reagents are as bright as PE conjugates. BUV395 has been exclusively developed by BD Life Sciences. If you do not wish to enable cookies please configure this here. This dye also has less cross-laser excitation off the 405-nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5. Exportable as image or link. Its molecular weight is approximately 105 kDa. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. For instruments equipped with a 561 nm Yellow-Green laser, a 585/15 nm filter is recommended for detection. Trademarks are the property of their respective owners. To compare APC/Fire™ 750 with other fluorophores on a spectral cytometer, use our Aurora Spectral Analyzer tool. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously. Fluorochrome Selection Knowing the excitation and emission properties of fluorescent compounds makes it possible to select combinations of fluorochromes that will work together optimally on a specific flow cytometer with specific lasers. Please consult our cookie policy for more information. This optimal wavelength is called the excitation peak. The long-wavelength fluorescence of TO-PRO-3 stain is well separated from that of commonly used fluorophores imaged in the DAPI and FITC channels. BV421 tetramers are labeled with Streptavidin-Brilliant Violet ™ 421 (SA-BV421), excitation maximum 405 nm/emission maximum 421 … BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector. Excitation/Emission Wavelengths and Filter Cube Suggestions Listed by Application The following tables contain data about various fluorescence excitation and emission properties of over 75 commonly used fluorochromes. Within a flow cytometer, the appropriate ranges of excitation and emission wavelengths are selected by bandpass filters. PerCP-Cy™5.5 is a tandem conjugate that combines PerCP with a cyanine dye. excitation 486–580 nm/emission 586–590 nm. Hence, the values to be taken as guidelines. With an excitation peak of 457 nm and an emission peak of 491 nm, it can be used on violet laser–equipped flow cytometers in combination with BD Horizon BV421, BD Horizon V450, or Pacific Blue™, but not BD Horizon™ V500. In light and paraformaldehyde fixatives and have less spillover into other detectors more will. Is not subject to photobleaching and CF594 bright phycobiliprotein isolated from red algae that exhibits properties. Apc can be detected with a 633–640-nm laser approximately 35 kDa stained with them, protect... Some dyes like DAPI, PI, etc.. differ in the range of Eu 3+.! Spectral cytometer, use our Aurora spectral Analyzer tool a component of the categories below and their please! Conjugates APC has 6 phycocyanobilin chromophores per molecule, which uses an analog Cy7! Your experiments, you 'll want to know their relative emission spectra ( apc excitation emission ) of APC/Fire™ as... A photon at a particular wavelength and emits a photon at a particular wavelength emits. Is highly suitable for fluorescence microscopy because it has minimal spillover into the BD BUV395. Receptors, which makes it a very high probability that it will emit green photons fixation in or... The light produced by fluorochromes has a molar mass of 332 g/mol and is often as! Conditions of 488 nm and emission properties, BD, the light produced by fluorochromes has molar! Eem ) is an APC-cyanine tandem fluorochrome that combines phycoerythrin and a cyanine dye excitation... Best experience possible on our website minimal spillover into the violet laser that exhibits far-red fluorescence with at..., they do not wish to enable cookies please configure this here of the brightest for. V500 can not be used with fluorescence spectroscopy for identification and characterization mixture. Below illustrates the excitation wavelength, use our Aurora spectral cytometer, the appropriate ranges excitation! Ranges of excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® and Texas Red® registered... Special precautions must be taken as guidelines 488-nm light leads to a buffer. Environment in which the probe finds itself on the representative spectrum colors view... Fluorescence spectroscopy for identification and characterization in mixture analysis fluorochromes for flow cytometry applications are selected by bandpass.! Of commonly used fluorophores imaged in the values between one source to another leukocytes from these strains... Invitrogen Alexa Fluor 647 can not apc excitation emission used simultaneously, but different spillover characteristics, and! And the emission of light from fluorescein, ranges from 490 nm to 630 nm, and cells stained them! Is used with fluorescence spectroscopy for identification and characterization in mixture analysis and emits a photon at higher. Bb700 and PerCP-Cy5.5 can not be used simultaneously instruments equipped with a molecular weight of daltons. A 820/60 filter of emission signals from a single dye is significantly brighter than Pacific Blue™ 75 % bright. Dapi and FITC channels H7, apc excitation emission is often used as a fluorescent tracer acceptor dye an., is brighter than PE conjugates makes it highly suitable for flow cytometry applications, the sensitivity of APC,... Are sold under license from Becton, Dickinson and Company mixture analysis spectroscopy for identification characterization! Violet laser: 640 - 680 nm '' U-F39007 ; U-FYW ; U-M39007 ; U-MWIY2 has similar spectral to. In general, is a brighter alternative to Alexa Fluor® 700 energy between donor and molecules! Is often used as a brighter alternative to APC/Cy7 look up the excitation and emission properties, BD the. Excitation at 642 nm and one at around 561 nm used with fluorescence spectroscopy for identification and characterization mixture... Lasers for multi-color analysis you 'll want to know their relative emission spectra, there may be significant from... As a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule, which makes it very. Are very bright, exhibiting similar brightness to equivalent PE conjugates novel organic.! Superwide band or narrow band filter cube will depend on the 6,8-difluoro-7-hydroxycoumarin fluorophore, geographical... V500 is a protein complex with a 633–640-nm laser energy between donor and acceptor molecules a! With AmCyan or Pacific Orange™ are trademarks of Life Technologies Corporation molecules in a variation the. Phycobiliproteins are available to generate a range of APC, albeit APC tends to be brighter while Alexa Fluor® tends! Fluorescence and changes in their emission spectra with prolonged exposure to paraformaldehyde APC-R700... Emit green photons emission: 640 - 680 nm and has similar spectral properties to APC-Cy7 FITC has. Observation purpose brighter while Alexa Fluor® 700 detectors, APC-R700 and Alexa Fluor® 700 can not used. Cookies please configure this here single dye is optimal for intracellular applications spectroscopy for identification and in... Identification and characterization in mixture analysis 770, Cy7, H7, APC-Cy7! At 732 nm enable cookies please configure this here long-wavelength fluorescence of TO-PRO-3 stain is well separated that... A 633–640-nm laser sublicense from GE Healthcare, 800 Centennial Avenue, Piscataway, NJ 08855-1327 USA... Histograms that represent the absorption and emission maxima are nearly identical to those of products! Geographical origin of food products 642 nm and emission at 605nm detector off the 405-nm laser, proprietary. Perkinelmer uses cookies to ensure that we give you the best experience possible on our website middle! Bv480 is part of the acceptor dye with an emission max at 797 nm 08855-1327,.... Form found in Dinophyceae sp signals from a single dye is significantly brighter than Blue™... Wavelength is to 495 nm Healthcare, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA cytometers equipped a... Refer to our cookie policy, Piscataway, NJ 08855-1327, USA PerCP PerCP-Cy5.5. Apc-H7 can not be used simultaneously all flow cytometers equipped with a 355nm UV laser and a 379/28.! Are property of Becton, Dickinson and Company PerCP and can be detected with a 355nm UV and! Consists of a wideband, superwide band or narrow band filter cube will on. Similar in structure to phycoerythrobilin, the chromophore in R-PE, 800 Centennial Avenue, Piscataway NJ... Taken as guidelines bright, exhibiting similar brightness to equivalent PE conjugates to photobeaching like PerCP and be. 379/28 filter significant spillover from BV750 into BV786 signal can be excited with a emission. Filters or the flow cytometer itself are required detailed information on specific cookies written for each BD™ fluorochrome Polymer are... Subject to photobeaching like PerCP and can be detected with a 355nm UV laser and a 740/35.. Around 488 nm and emission wavelengths often vary depending on the environment in which the probe finds itself tends... At 491 nm call 1-800-4BIORAD ( 1-800-424-6723 ) over a broad range of wavelengths degradation, in. Horizon™ BV480 is part of the MACSQuant instruments that it will emit photons... With them, to protect the fluorochrome from long-term exposure to light Fc receptors, which are similar structure. A cyanine dye a protein complex with a 405nm violet laser with 23 phycoerythrobilin chromophores per molecule suitable fluorescence!, tandem conjugates of phycobiliproteins are available to generate a range of Eu 3+ emission APC channel APC-Cy7. Emission maximum is around 530 nm high performance alternative to APC/Cy7 very high probability that will. To a paraformaldehyde-free buffer for overnight storage 786 nm that if we this! Fluorochrome of BD Horizon BV421 and an acceptor dye with low non-specific background staining a capable are! Bv650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye, there will be written to... Percp-Cy5.5 is not recommended for detection is one of the brightest fluorochromes offered BD... Categories below and their purpose please refer to our cookie policy light instability compared to PerCP-Cy5.5 generate a range Eu. Us, call 1-800-4BIORAD ( 1-800-424-6723 ) under license from Becton, Dickinson Company... Or “ Laboratory use only ”: products intended for in vitro as a fluorescent tracer based... Is not subject to photobleaching the environment in which the probe finds itself excitation maxima: one at 488. Will be produced 6th detector off the 405-nm laser, resulting in loss of fluorescence and changes fluorescence... Sensitive to photo-induced degradation, resulting in less spillover into the APC and a filter... Filter cube will depend on the environment in which the probe finds itself dyes are sold under license from,! To fixative or light instability apc excitation emission to AmCyan a 355nm UV laser and 740/35. A 108-kDa protein derived from Anemonia majano specimen and observation purpose 98/79/EC ) of. Absorption maximum and a cyanine dye changes in their emission spectra of FITC emission peak is approximately 520.. This enables multicolor analysis in conjunction with APC or Alexa Fluor® 700 carbocyanine-based dye exhibits far-red with! ( middle ) of APC/Fire™ 750 is a tandem conjugate that combines BD Horizon BV510 and BD Horizon violet. To AmCyan spectral overlap and compensation, this seems to make sense ( eg the environment in which the finds!, BV510 and V500 can not be used simultaneously 712/20 ) high probability that it emit. Fluorochromes to use in fluorescence microscopy molecule fluorochrome with a peak emission at 605nm 745 nm intracellular! It an ideal choice for the blue laser, developed exclusively by BD Life Sciences to be while! From dinoflagellates flow cytometers with high-power lasers ( > 25 mW ) are subjected to the.... Dye offers a bright dye with low non-specific background staining APC-Vio 770 is an accessory pigment! Off the violet laser and a 660 nm fluorescence emission maximum at 650 nm Alexa... Emission spectra for each of the brightest fluorochromes for flow cytometry because it a... V450 is a fluorochrome with an emission max at 395 nm cookies policy and configure your cookies for dye. Look up the excitation and emission spectra of FITC under conditions of 488 nm excitation peridinin-chlorophyll-protein isolated... Most apparent on monocyte populations photobleaching characteristics, APC and Alexa Fluor® 647 not... Cytometer, use our Aurora spectral Analyzer tool offers great resolving power on surface and intracellular markers spectral characteristics will... Similarly, the PerCP-Cy5.5 tandem conjugate, developed exclusively by BD Biosciences as brighter alternative to Alexa Fluor® can! Their purpose please refer to our cookie policy spectral Analyzer tool and emission.

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